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CyFlow™ Helios PE

CyFlow™ Helios PE
Application: Flow cytometry
Emission Maximum: 576 nm
Alternative Name: IKZF2
Antigen: Helios
Field of Interest: Transcription
Source Species: Hamster
Isotype: IgG
Target Species: Human, Mouse
Excitation Maximum: 496 nm, 565 nm
Clonality: monoclonal
Laser: Blue, Green, Yellow
Format/Fluorochrome: PE
Regulatory Status: RUO
Clone: 22F6
Order number: CV254145
Quantity 100 tests Volume 1.0 mL Immunogen Peptide coresponding to the amino acids... more
Product information "CyFlow™ Helios PE"
Quantity100 tests
Volume1.0 mL
ImmunogenPeptide coresponding to the amino acids 51-107 of Helios
Background InformationHelios (IKZF2; Ikaros family zinc finger protein 2) is a hematopoietic-specific transcription factor involved in the regulation of lymphocyte development, together with other members of this family, such as Aiolos and Ikaros. Helios forms homo- and heterodimers with these proteins and is thought to function predominantly in early hematopoietic development. Expression of Helios, Aiolos and Ikaros is restricted to cells of the hematopoietic system, whereas other family members, Eos and Pegassus, are more widely expressed. Helios is expressed at early stages of thymocyte development. In mature T cells, Helios has been strongly associated with Treg cells.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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