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CyFlow™ CD43 Pacific Blue™

CyFlow™ CD43 Pacific Blue™
Antigen: CD43
Alternative Name: Leukosialin, sialophorin
Clone: MEM-59
Application: Flow cytometry
Format/Fluorochrome: Pacific Blue™
Laser: Violet
Target Species: Human
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 455 nm
Excitation Maximum: 400 to 410 nm
Isotype: IgG1
Order number: CC607958

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

HLDA Workshop HLDA IV—WS Code NL 604 HLDA V—WS Code AS S290 Quantity 100 tests Volume... more
CyFlow™ CD43 Pacific Blue™
HLDA WorkshopHLDA IV—WS Code NL 604 HLDA V—WS Code AS S290
Quantity100 tests
Volume0.4
ImmunogenHuman T lymphocytes
Background InformationCD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation. By interaction with actin-binding proteins ezrin and moesin, CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic. CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 causes induction of functionally active tumor suppressor p53 protein, but in case of p53 and ARF defficiency CD43 promotes tumour proliferation and viability. It appears to be an important modulator of leukocyte functions.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Stefanova I, Hilgert I, Angelisova P, Kristofova H, Horejsi V: Characterization of a 95 kDa human leucocyte sialoglycoprotein: its identity with CD43, gpL115, leukosialin and sialophorin. Folia·Biol·(Praha). 1988; 34(4):255‑65. <·PMID:·2467832·> | Knapp W, Dorken B, Gilks W, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (Eds): Leucocyte Typing IV. Oxford·University·Press,·Oxford. 1989; 1‑1820. <·NLM·ID:·8914679·> | Stefanova I, Hilgert I, Kristofova H, Brown R, Low MG, Horejsi V: Characterization of a broadly expressed human leucocyte surface antigen MEM‑43 anchored in membrane through phosphatidylinositol. Mol·Immunol. 1989·Feb; 26(2):153‑61. <·PMID:·2918859·> | Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J·Clin·Pathol. 1993·Apr; 46(4):334‑6. <·PMID:·7684403·> | Alvarado M, Klassen C, Cerny J, Horejsi V, Schmidt RE: MEM‑59 monoclonal antibody detects a CD43 epitope involved in lymphocyte activation. Eur·J·Immunol. 1995·Apr; 25(4):1051‑5. <·PMID:·7537669·> | Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland·Publishing·Inc,·New·York. 1997; 1‑1342. <·NLM·ID:·9712219·> | Cermák L, Símová S, Pintzas A, Horejsí V, Andera L: Molecular mechanisms involved in CD43‑mediated apoptosis of TF‑1 cells: Roles of transcription Daxx expression, and adhesion molecules. J·Biol·Chem. 2002·Mar·8; 277(10):7955‑61. <·PMID:·11773067·> | Kadaja L, Laos S, Maimets T: Overexpression of leukocyte marker CD43 causes activation of the tumor suppressor proteins p53 and ARF. Oncogene. 2004·Apr·1; 23(14):2523‑30. <·PMID:·14676827·> | Filatov AV, Krotov GI, Zgoda VG, Volkov Y: Fluorescent immunoprecipitation analysis of cell surface proteins: a methodology compatible with mass‑spectrometry. J·Immunol·Methods. 2007·Jan·30; 319(1‑2):21‑33. <·PMID:·17098248·> | Mody PD, Cannon JL, Bandukwala HS, Blaine KM, Schilling AB, Swier K, Sperling AI: Signaling through CD43 regulates CD4 T‑cell trafficking. Blood. 2007·Oct·15; 110(8):2974‑82. <·PMID:·17638845·> | Khunkaewla P, Schiller HB, Paster W, Leksa V, Cermák L, Anděra L, Hořejší V, Stockinger H: LFA‑1‑mediated leukocyte adhesion regulated by interaction of CD43 with LFA‑1 and CD147. Mol·Immunol. 2007·Nov·8; <·PMID:·19203795·> | Simova S, Klima M, Cermak L, Sourkova V, Andera L: Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL‑R1/DR4 to the plasma membrane. Apoptosis. 2008·Mar; 13(3):423‑36. <·PMID:·18165900·> | Kadaja-Saarepuu L, Laos S, Jääger K, Viil J, Balikova A, Lõoke M, Hansson GC, Maimets T Skip to main content Skip to navigation Resources How To About NCBI Accesskeys Sign in to NCBI PubMed US National Library of Medicine National Institutes of Health Search databaseSearch termClear inputSearch Create RSSCreate alertAdvancedHelp Result Filters AbstractSend to: Oncogene. 2008 Mar 13;27(12):1705-15. Epub 2007 Sep 24. CD43 promotes cell growth and helps to evade FAS-mediated apoptosis in non-hematopoietic cancer cells lacking the tumor suppressors p53 or ARF. Kadaja-Saarepuu L1, Laos S, Jääger K, Viil J, Balikova A, Lõoke M, Hansson GC, Maimets T.: CD43 promotes cell growth and helps to evade FAS‑mediated apoptosis in non‑hematopoietic cancer cells lacking the tumor suppressors p53 or ARF. Oncogene. 2008·Mar·13; 27(12):1705‑15. <·PMID:·17891181·>