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CyFlow™ CD25 FITC

CyFlow™ CD25 FITC
Antigen: CD25
Alternative Name: IL2Rα, p55, TAC
Clone: PC61.5
Application: Flow cytometry
Format/Fluorochrome: FITC
Laser: Blue
Target Species: Mouse
Field of Interest: Immunophenotyping, MHC
Species of Origin: Rat
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 518 nm
Excitation Maximum: 490 to 495 nm
Isotype: IgG1
Order number: BG913987

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

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*1 Offer valid until 31/10/2017
Concentration Unit mg/mL Concentration 0.5 Quantity 0.1 mg Volume 0.2... more
CyFlow™ CD25 FITC
Concentration Unitmg/mL
Concentration0.5
Quantity0.1 mg
Volume0.2
ImmunogenB6.1 CTL cell line
Background InformationCD25 (IL2Rα, Tac) is a ligand-binding α subunit of interleukin 2 receptor (IL2R). Together with β and γ subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. CD25+ CD4+ FoxP3+ regulatory cells (Treg cells) play a crucial role in the control of organ-specific autoimmune diseases.
UsageThe reagent is designed for Flow Cytometry analysis. Working concentrations should be determined by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Aiba Y, Hirayama F, Ogawa M: Clonal proliferation and cytokine requirement of murine progenitors for natural killer cells. Blood. 1997·Jun·1; 89(11):4005‑12. <·PMID:·9166839·> | Jones CM, Cose SC, Coles RM, Winterhalter AC, Brooks AG, Heath WR, Carbone FR: Herpes simplex virus type 1‑specific cytotoxic T‑lymphocyte arming occurs within lymph nodes draining the site of cutaneous infection. J·Virol. 2000·Mar; 74(5):2414‑9. <·PMID:·10666272·> | Yu CT, Feng MH, Shih HM, Lai MZ: Increased p300 expression inhibits glucocorticoid receptor‑T‑cell receptor antagonism but does not affect thymocyte positive selection. Mol·Cell·Biol. 2002·Jul; 22(13):4556‑66. <·PMID:·12052865·> | Kish DD, Gorbachev AV, Fairchild RL: CD8+ T cells produce IL‑2, which is required for CD(4+)CD25+ T cell regulation of effector CD8+ T cell development for contact hypersensitivity responses. J·Leukoc·Biol. 2005·Sep; 78(3):725‑35. <·PMID:·16000396·> | Kotner J, Tarleton R: Endogenous CD4(+) CD25(+) regulatory T cells have a limited role in the control of Trypanosoma cruzi infection in mice. Infect·Immun. 2007·Feb; 75(2):861‑9. <·PMID:·17101658·> | Van VQ, Darwiche J, Raymond M, Lesage S, Bouguermouh S, Rubio M, Sarfati M: Cutting edge: CD47 controls the in vivo proliferation and homeostasis of peripheral CD4+ CD25+ Foxp3+ regulatory T cells that express CD103. J·Immunol. 2008·Oct·15; 181(8):5204‑8. <·PMID:·18832672·> | Wei HX, Chuang YH, Li B, Wei H, Sun R, Moritoki Y, Gershwin ME, Lian ZX, Tian Z: CD4+ CD25+ Foxp3+ regulatory T cells protect against T cell‑mediated fulminant hepatitis in a TGF‑beta‑dependent manner in mice. J·Immunol. 2008·Nov·15; 181(10):7221‑9. <·PMID:·18981144·> | Tenorio EP, Olguín JE, Fernández J, Vieyra P, Saavedra R: Reduction of Foxp3+ cells by depletion with the PC61 mAb induces mortality in resistant BALB/c mice infected with Toxoplasma gondii. J·Biomed·Biotechnol. 2010; 2010:786078. <·PMID:·20037737·>