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CyFlow™ CD3 PE-Cy7

CyFlow™ CD3 PE-Cy7
Antigen: CD3
Alternative Name: Leu4, T3
Clone: SK7
Application: Flow cytometry
Format/Fluorochrome: PE-Cy7
Laser: Blue, Green, Yellow
Target Species: Human, Non-Human Primates
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 785 nm
Excitation Maximum: 496 nm, 565 nm
Isotype: IgG1
Order number: BQ006366

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

HLDA Workshop HLDA II—WS Code T118 HLDA III—WS Code T492 Quantity 100 tests Volume... more
CyFlow™ CD3 PE-Cy7
HLDA WorkshopHLDA II—WS Code T118 HLDA III—WS Code T492
Quantity100 tests
Volume0.4
ImmunogenHuman thymocytes
Background InformationCD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3γ, CD3 δ, CD3ε and CD3ζ. These CD3 subunits are structurally related members of the immunoglobulins superfamily encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Wood GS, Burns BF, Dorfman RF, Warnke RA: The immunohistology of non‑T cells in the acquired immunodeficiency syndrome. Am·J·Pathol. 1985·Sep; 120(3):371‑9. <·PMID:·3898858·> | Li B, Wang H, Dai J, Ji J, Qian W, Zhang D, Hou S, Guo Y: Construction and characterization of a humanized anti‑human CD3 monoclonal antibody 12F6 with effective immunoregulation functions. Immunology. 2005·Dec; 116(4):487‑98. <·PMID:·16313362·> | Recio MJ, Moreno-Pelayo MA, Kiliç SS, Guardo AC, Sanal O, Allende LM, Pérez-Flores V, Mencía A, Modamio-Høybjør S, Seoane E, Regueiro JR: Differential biological role of CD3 chains revealed by human immunodeficiencies. J·Immunol. 2007·Feb·15; 178(4):2556‑64. <·PMID:·17277165·> | Guttman-Yassky E, Vugmeyster Y, Lowes MA, Chamian F, Kikuchi T, Kagen M, Gilleaudeau P, Lee E, Hunte B, Howell K, Dummer W, Bodary SC, Krueger JG: Blockade of CD11a by efalizumab in psoriasis patients induces a unique state of T‑cell hyporesponsiveness. J·Invest·Dermatol. 2008·May; 128(5):1182‑91. <·PMID:·18239614·>