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CyFlow™ BrdU PE

CyFlow™ BrdU PE
Application: Flow cytometry
Clonality: monoclonal
Regulatory Status: RUO
Laser: Blue, Green, Yellow
Source Species: Mouse
Format/Fluorochrome: PE
Excitation Maximum: 496 nm, 565 nm
Emission Maximum: 576 nm
Isotype: IgG1
Antigen: 5-bromodeoxyuridine
Clone: Bu20a
Field of Interest: Small organic molecules
Order number: CL205376

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

Quantity 100 tests Volume 1.0 mL Immunogen 5-bromodeoxyuridine conjugated to BSA... more
CyFlow™ BrdU PE
Quantity100 tests
Volume1.0 mL
Immunogen5-bromodeoxyuridine conjugated to BSA
Background Information5-bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Magaud JP, Sargent I, Clarke PJ, Ffrench M, Rimokh R, Mason DY: Double immunocytochemical labeling of cell and tissue samples with monoclonal anti‑bromodeoxyuridine. J·Histochem·Cytochem. 1989·Oct; 37(10):1517‑27. <·PMID:·2476478·> | Muskhelishvili L, Latendresse JR, Kodell RL, Henderson EB: Evaluation of cell proliferation in rat tissues with BrdU, PCNA, Ki‑67(MIB‑5) immunohistochemistry and in situ hybridization for histone mRNA. J·Histochem·Cytochem. 2003·Dec; 51(12):1681‑8. <·PMID:·14623936·> | Martín CA, Surur JM, García MN, Corrons F, Badrán AF: Comparative study of DNA synthesis and nucleolar organizer regions of sinusoid littoral cells in mouse regenerating liver. Biocell. 2005·Apr; 29(1):33‑8. <·PMID:·15954465·> | Shih CH, Whalen BA, Goto Y, Hogg JC, van Eeden SF: Flow cytometric method for enumeration and characterization of newly released polymorphonuclear leukocytes from the bone marrow using 5'‑bromo‑2'‑deoxyuridine. Am·J·Physiol·Cell·Physiol. 2005·Sep; 289(3):C757‑65. <·PMID:·15857903·> | Pajalunga D, Mazzola A, Salzano AM, Biferi MG, De Luca G, Crescenzi M: Critical requirement for cell cycle inhibitors in sustaining nonproliferative states. J·Cell·Biol. 2007·Mar·12; 176(6):807‑18. <·PMID:·17353358·> | Lagares A, Li HY, Zhou XF, Avendaňo C: Primary sensory neuron addition in the adult rat trigeminal ganglion: evidence for neural crest glio‑neuronal precursor maturation. J·Neurosci. 2007·Jul·25; 27(30):7939‑53. <·PMID:·17652585·> | Yatera K, Hsieh J, Hogg JC, Tranfield E, Suzuki H, Shih CH, Behzad AR, Vincent R, van Eeden SF: Particulate matter air pollution exposure promotes recruitment of monocytes into atherosclerotic plaques. Am·J·Physiol·Heart·Circ·Physiol. 2008·Feb; 294(2):H944‑53. <·PMID:·18083905·> | O'Driscoll M, Jeggo PA: CsA can induce DNA double‑strand breaks: implications for BMT regimens particularly for individuals with defective DNA repair. Bone·Marrow·Transplant. 2008·Jun; 41(11):983‑9. <·PMID:·18278071·>