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CyFlow™ CD46 Alexa Fluor™ 700

CyFlow™ CD46 Alexa Fluor™ 700
Application: Flow cytometry
Clonality: monoclonal
Target Species: Cow, Human
Regulatory Status: RUO
Source Species: Mouse
Field of Interest: Immunophenotyping
Laser: Red
Isotype: IgG1
Format/Fluorochrome: Alexa Fluor™ 700
Excitation Maximum: 702 nm
Emission Maximum: 723 nm
Antigen: CD46
Alternative Name: MCP
Clone: MEM-258
Order number: CG141895

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

Quantity 100 tests Volume 0.4 mL Immunogen Human Acute Lymphocytic Leukemia (ALL) T... more
CyFlow™ CD46 Alexa Fluor™ 700
Quantity100 tests
Volume0.4 mL
ImmunogenHuman Acute Lymphocytic Leukemia (ALL) T cells
Background InformationCD46 (MCP; membrane cofactor protein) is a multifunctional cell surface transmembrane protein that binds and inactivates C3b and C4b complement fragments, regulates T cell-induced inflammatory responses by either inhibiting (CD46-1 isoform) or increasing (CD46-2 isoform) the contact hypersensitivity reaction. CD46 also serves as a receptor for several human pathogens (both bacteria and viruses), and its ligation alteres T lymphocyte polarization toward antigen-presenting cells or target cells, inhibiting lymphocyte function. CD46 is a protector of placental tissue and is also expressed on the inner acrosomal membrane of spermatozoa.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Okada N, Liszewski MK, Atkinson JP, Caparon M: Membrane cofactor protein (CD46 is a keratinocyte receptor for the M protein of the group A streptococcus. Proc·Natl·Acad·Sci·USA. 1995·Mar·28; 92(7):2489‑93. <·PMID:·7708671·> | Marie JC, Astier AL, Rivailler P, Rabourdin-Combe C, Wild TF, Horvat B: Linking innate and acquired immunity: divergent role of CD46 cytoplasmic domains in T cell induced inflammation. Nat·Immunol. 2002·Jul; 3(7):659‑66. <·PMID:·12055630·> | Gaggar A, Shayakhmetov DM, Lieber A: CD46 is a cellular receptor for group B adenoviruses. Nat·Med. 2003·Nov; 9(11):1408‑12. <·PMID:·14566335·> | Fleischli C, Verhaagh S, Havenga M, Sirena D, Schaffner W, Cattaneo R, Greber UF, Hemmi S: The distal short consensus repeats 1 and 2 of the membrane cofactor protein CD46 and their distance from the cell membrane determine productive entry of species B adenovirus serotype 35. J·Virol. 2005·Aug; 79(15):10013‑22. <·PMID:·16014961·> | Liszewski MK, Kemper C, Price JD, Atkinson JP: Emerging roles and new functions of CD46. Springer·Semin·Immunopathol. 2005·Nov; 27(3):345‑58. <·PMID:·16200405·> | Weyand NJ, Lee SW, Higashi DL, Cawley D, Yoshihara P, So M: Monoclonal antibody detection of CD46 clustering beneath Neisseria gonorrhoeae microcolonies. Infect·Immun. 2006·Apr; 74(4):2428‑35. <·PMID:·16552073·> | Fremeaux-Bacchi V, Moulton EA, Kavanagh D, Dragon-Durey MA, Blouin J, Caudy A, Arzouk N, Cleper R, Francois M, Guest G, Pourrat J, Seligman R, Fridman WH, Loirat C, Atkinson JP: Genetic and functional analyses of membrane cofactor protein (CD46) mutations in atypical hemolytic uremic syndrome. J·Am·Soc·Nephrol. 2006·Jul; 17(7):2017‑25. <·PMID:·16762990·> | Oliaro J, Pasam A, Waterhouse NJ, Browne KA, Ludford-Menting MJ, Trapani JA, Russell SM: Ligation of the cell surface receptor, CD46, alters T cell polarity and response to antigen presentation. Proc·Natl·Acad·Sci·USA. 2006·Dec·5; 103(49):18685‑90. <·PMID:·17116876·> | Loré K, Adams WC, Havenga MJ, Precopio ML, Holterman L, Goudsmit J, Koup RA: Myeloid and plasmacytoid dendritic cells are susceptible to recombinant adenovirus vectors and stimulate polyfunctional memory T cell responses. J·Immunol. 2007·Aug·1; 179(3):1721‑9. <·PMID:·17641038·> | Fleischli C, Sirena D, Lesage G, Havenga MJ, Cattaneo R, Greber UF, Hemmi S: Species B adenovirus serotypes 3, 7, 11 and 35 share similar binding sites on the membrane cofactor protein CD46 receptor. J·Gen·Virol. 2007·Nov; 88(11):2925‑34. <·PMID:·17947513·> | Hoffmann D, Bayer W, Heim A, Potthoff A, Nettelbeck DM, Wildner O: Evaluation of twenty‑one human adenovirus types and one infectivity‑enhanced adenovirus for the treatment of malignant melanoma. J·Invest·Dermatol. 2008·Apr; 128(4):988‑98. <·PMID:·17960177·> | Wang H, Tuve S, Erdman DD, Lieber A: Receptor usage of a newly emergent adenovirus type 14. Virology. 2009·May·10; 387(2):436‑41. <·PMID:·19307010·> | Rebetz J, Na M, Su C, Holmqvist B, Edqvist A, Nyberg C, Widegren B, Salford LG, Sjögren HO, Arnberg N, Qian Q, Fan X: Fiber mediated receptor masking in non‑infected bystander cells restricts adenovirus cell killing effect but promotes adenovirus host co‑existence. PLoS·One. 2009·Dec·29; 4(12):e8484. <·PMID:·20041185·> | Kälin S, Amstutz B, Gastaldelli M, Wolfrum N, Boucke K, Havenga M, DiGennaro F, Liska N, Hemmi S, Greber UF: Macropinocytotic uptake and infection of human epithelial cells with species B2 adenovirus type 35. J·Virol. 2010·May; 84(10):5336‑50. <·PMID:·20237079·>