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CyFlow™ CD45RA FITC

CyFlow™ CD45RA FITC
Application: Flow cytometry
Clonality: monoclonal
Format/Fluorochrome: FITC
Target Species: Human
Regulatory Status: RUO
Isotype: IgG2b
Laser: Blue
Excitation Maximum: 490 to 495 nm
Emission Maximum: 518 nm
Source Species: Mouse
Field of Interest: Immunophenotyping, Phosphorylation
Antigen: CD45RA
Alternative Name: Leu18
Clone: MEM-56
Order number: AA378227
HLDA Workshop HLDA IV—WS Code NL 907 Quantity 100 tests Volume 2.0 mL Immunogen... more
Product information "CyFlow™ CD45RA FITC"
HLDA WorkshopHLDA IV—WS Code NL 907
Quantity100 tests
Volume2.0 mL
ImmunogenHuman thymocytes and T lymphocytes
Background InformationCD45RA (Leu18) is a high molecular weight isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RA is expressed e.g. on naive T cells and normal plasma cells.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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* All prices are quoted net of the statutory value-added tax and shipping costs and possibly delivery charges, if not otherwise described

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