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CyFlow™ CD45 Purified

CyFlow™ CD45 Purified
Antigen: CD45
Alternative Name: B220, LCA, T200
Clone: 2D1
Application: Flow cytometry
Format/Fluorochrome: Purified
Target Species: Human
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Isotype: IgG1
Order number: AA542037

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

HLDA Workshop HLDA III Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg... more
CyFlow™ CD45 Purified
Concentration Unitmg/mL
Quantity0.1 mg
ImmunogenHuman peripheral blood mononuclear cells (PBMC)
Background InformationCD45 (LCA; leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Pfau JC, Walker E, Card GL: Monoclonal antibodies to CD45 modify LPS‑induced arachidonic acid metabolism in macrophages. Biochim·Biophys·Acta. 2000·Feb·28; 1495(3):212‑22. <·PMID:·10699460·> | Janossy G, Jani IV, Bradley NJ, Bikoue A, Pitfield T, Glencross DK: Affordable CD4(+)‑T‑cell counting by flow cytometry: CD45 gating for volumetric analysis. Clin·Diagn·Lab·Immunol. 2002·Sep; 9(5):1085‑94. <·PMID:·12204964·> | Wellhausen SR, Slone SP, Miller JJ: Clone‑specific anti‑CD45 blocking factor in patient plasma. Cytometry·B·Clin·Cytom. 2007·Sep; 72(5):423‑6. <·PMID:·17311353·> | Ito A, Ishida T, Yano H, Inagaki A, Suzuki S, Sato F, Takino H, Mori F, Ri M, Kusumoto S, Komatsu H, Iida S, Inagaki H, Ueda R: Defucosylated anti‑CCR4 monoclonal antibody exercises potent ADCC‑mediated antitumor effect in the novel tumor‑bearing humanized NOD/Shi‑scid, IL‑2Rgamma(null) mouse model. Cancer·Immunol·Immunother. 2009·Aug; 58(8):1195‑206. <·PMID:·19048251·> | Mariucci S, Rovati B, Bencardino K, Manzoni M, Danova M: Flow cytometric detection of circulating endothelial cells and endothelial progenitor cells in healthy subjects. Int·J·Lab·Hematol. 2010·Feb; 32(1‑1):e40‑8. <·PMID:·20088999·> | Vicetti Miguel RD, Harvey SA, LaFramboise WA, Reighard SD, Matthews DB, Cherpes TL: Human female genital tract infection by the obligate intracellular bacterium Chlamydia trachomatis elicits robust Type 2 immunity. PLoS·One. 2013; 8(3):e58565. <·PMID:·23555586·>