CyFlow™ CD2 Pacific Blue™
| Antigen: | CD2 |
| Alternative Name: | LFA-2 |
| Clone: | LT2 |
| Application: | Flow cytometry |
| Format/Fluorochrome: | Pacific Blue™ |
| Laser: | Violet |
| Target Species: | Human |
| Field of Interest: | Immunophenotyping |
| Species of Origin: | Mouse |
| Regulatory Status: | RUO |
| Clonality: | monoclonal |
| Emission Maximum: | 455 nm |
| Excitation Maximum: | 400 to 410 nm |
| Isotype: | IgG2b |
| HLDA Workshop | HLDA VI—WS Code T 6T-008 |
| Quantity | 100 tests |
| Volume | 0.4 |
| Immunogen | Normal human blood lymphocytes |
| Background Information | CD2 (LFA-2) belongs to T lymphocyte glycoproteins of immunoglobulin superfamily. Its interaction with CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Filatov AV, Bachurin PS, Markova NA, Surnakova NE: A panel of monoclonal antibodies against human lymphocyte antigens. Eksp·Onkol. 1989; 11(2):28‑32. <·PMID:·2737128·> | Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland·Publishing·Inc,·New·York. 1997; 1‑1342. <·NLM·ID:·9712219·> | Dustin ML: Adhesive bond dynamics in contacts between T lymphocytes and glass‑supported planar bilayers reconstituted with the immunoglobulin‑related adhesion molecule CD58. J·Biol·Chem. 1997·Jun·20; 272(25):15782‑8. <·PMID:·9188475·> | Gannon GA, Rhind SG, Suzui M, Zamecnik J, Sabiston BH, Shek PN, Shephard RJ: beta‑Endorphin and natural killer cell cytolytic activity during prolonged exercise: Is there a connection?. Am·J·Physiol. 1998·Dec; 275(6‑2):R1725‑34. <·PMID:·9843861·> | Lin CW, Liu TY, Chen SU, Wang KT, Medeiros LJ, Hsu SM: CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features. Blood. 2005·Nov·15; 106(10):3567‑74. <·PMID:·16046525·> | Zhu DM, Dustin ML, Cairo CW, Thatte HS, Golan DE: Mechanisms of Cellular Avidity Regulation in CD2‑CD58‑Mediated T Cell Adhesion. ACS·Chem·Biol. 2006·Nov·21; 1(10):649‑58. <·PMID:·17168569·> | Pawlowski NN, Struck D, Grollich K, Kuhl AA, Zeitz M, Liesenfeld O, Hoffmann JC: CD2 deficiency partially prevents small bowel inflammation and improves parasite control in murine Toxoplasma gondii infection. World·J·Gastroenterol. 2007·Aug·21; 13(31):4207‑13. <·PMID:·17696249·>
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