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CyFlow™ CD82 Low Endotoxin

CyFlow™ CD82 Low Endotoxin
Antigen: CD82
Alternative Name: C33, R2l 4F9
Clone: C33
Application: Flow cytometry, Functional assays, Immunocytochemistry, Immunohistochmistry (paraffin-embedded sections), Immunoprecipitation, Western blot
Format/Fluorochrome: Low Endotoxin
Target Species: Human
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Isotype: IgG2a
Order number: BV965722

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

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*1 Offer valid until 31/10/2017
Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 Immunogen... more
CyFlow™ CD82 Low Endotoxin
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1
ImmunogenC91/PL (human HTLV-1+ T cell line)
Background InformationCD82 (KAI1), a member of the tetraspanin family, forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules. These complexes influence adhesion, morphology, activation, proliferation and differentiation of B, T and other cells. CD82 regulates cytoskeleton rearrangement and may participate in the turnover of the tetraspanin complex members. Besides in the plasma membrane, CD82 is localized also in endosome/lysosome compartments. Tumor-suppressive roles of CD82 have been demonstrated.
Storage BufferThe reagent is provided in azide-free phosphate buffered saline (PBS) solution, pH ≈7.4; 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Fukudome K, Furuse M, Imai T, Nishimura M, Takagi S, Hinuma Y, Yoshie O: Identification of membrane antigen C33 recognized by monoclonal antibodies inhibitory to human T‑cell leukemia virus type 1 (HTLV‑1)‑induced syncytium formation: altered glycosylation of C33 antigen in HTLV‑1‑positive T cells. J·Virol. 1992·Mar; 66(3):1394‑401. <·PMID:·1738199·> | Imai T, Yoshie O: C33 antigen and M38 antigen recognized by monoclonal antibodies inhibitory to syncytium formation by human T cell leukemia virus type 1 are both members of the transmembrane 4 superfamily and associate with each other and with CD4 or CD8 in T cells. J·Immunol. 1993·Dec·1; 151(11):6470‑81. <·PMID:·8245480·> | Imai T, Kakizaki M, Nishimura M, Yoshie O: Molecular analyses of the association of CD4 with two members of the transmembrane 4 superfamily, CD81 and CD82. J·Immunol. 1995·Aug·1; 155(3):1229‑39. <·PMID:·7636191·> | Ueda T, Ichikawa T, Tamaru J, Mikata A, Akakura K, Akimoto S, Imai T, Yoshie O, Shiraishi T, Yatani R, Ito H, Shimazaki J: Expression of the KAI1 protein in benign prostatic hyperplasia and prostate cancer. Am·J·Pathol. 1996·Nov; 149(5):1435‑40. <·PMID:·8909232·> | Escola JM, Kleijmeer MJ, Stoorvogel W, Griffith JM, Yoshie O, Geuze HJ: Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B‑lymphocytes. J·Biol·Chem. 1998·Aug·7; 273(32):20121‑7. <·PMID:·9685355·>