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CyFlow™ CD73 FITC
| Application: | Flow cytometry |
| Isotype: | IgG1 |
| Alternative Name: | E5NT, Ecto'-5-nucleotidase, eN, eNT, NT5, NTE |
| Antigen: | CD73 |
| Field of Interest: | Immunophenotyping |
| Clonality: | monoclonal |
| Source Species: | Mouse |
| Emission Maximum: | 518 nm |
| Excitation Maximum: | 490 to 495 nm |
| Laser: | Blue |
| Regulatory Status: | RUO |
| Target Species: | Human |
| Format/Fluorochrome: | FITC |
| Clone: | AD2 |
| Order number: | AY261195 |
For Research Use Only
Not for use in diagnostic or therapeutic procedures.
Quantity 100 tests Volume 0.4 mL Immunogen Not available Background Information... more
Product information "CyFlow™ CD73 FITC"
| Quantity | 100 tests |
| Volume | 0.4 mL |
| Immunogen | Not available |
| Background Information | CD73 (ecto-5´-nucleotidase) is a 70 kDa glycoprotein anchored to the extracellular leaflet of the plasma membrane by GPI. This ecto-enzyme catalyzes dephosphorylation of AMP to adenosine. CD73 is expressed in various types of cells, such as epithelial, muscle, and endothelial cells, neutrophils, lymphocytes and fibroblasts. Inflammatory mediators support CD73 expression and its enzymatic activity, leading to the release of adenosine, which modulates inflammation through adenosine receptors. CD73 is expressed in a variety of lymphomas and leukemias, including ALL and CLL, whereas immunodeficient patients usually express low levels of this protein. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
Technical Data Sheet
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