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CyFlow™ CD59 Alexa Fluor™ 647

CyFlow™ CD59 Alexa Fluor™ 647
Antigen: CD59
Alternative Name: Protectin H19
Clone: MEM-43
Application: Flow cytometry
Format/Fluorochrome: Alexa Fluor™ 647
Laser: Red
Target Species: Human
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 665 nm
Excitation Maximum: 650 nm
Isotype: IgG2a
Order number: BE560278

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

Quantity 100 tests Volume 0.4 ml Immunogen Thymocytes and T lymphocytes Specifity... more
CyFlow™ CD59 Alexa Fluor™ 647
Quantity100 tests
Volume0.4 ml
ImmunogenThymocytes and T lymphocytes
SpecifityThe mouse monoclonal antibody MEM-43 recognizes well defined epitope (W40, R-53) on CD59 antigen, an 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on all hematopoietic cells; it is widely present on cells in all tissues.
Background InformationCD59 (Protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). It is thus the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 associates with C5b-8 complex and thereby counteracts appropriate formation of cytolytic pore within the plasma membrane. CD59 is also an low-affinity ligand of human CD2 and causes T cell costimulation.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.1% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

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