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CyFlow™ CD53 Low Endotoxin

CyFlow™ CD53 Low Endotoxin
Antigen: CD53
Alternative Name: TSPAN2
Clone: MEM-53
Application: Flow cytometry, Functional assays, Immunohistochemistry (frozen sections), Immunoprecipitation, Western blot
Format/Fluorochrome: Low Endotoxin
Target Species: Human
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Isotype: IgG1
Order number: BY891522

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

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*1 Offer valid until 31/10/2017
Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 Immunogen... more
CyFlow™ CD53 Low Endotoxin
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1
ImmunogenLeukocytes of a patient suffering from LGL-type leukemia
Background InformationCD53 is a tetraspanin family transmembrane glycoprotein expressed in the lymphoid-myeloid lineage. This molecule has been reported to form complexes with other leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4 or tetraspanins CD37, CD81 and CD82, thus probably modulating various signaling processes. CD53 is involved in radioresistancy of tumor cells and its triggering has anti-apoptotic effect. In thymus, CD53 is up-regulated in response to positive selection signals during T cell development, and is strongly expressed upon macrophage exposure to bacterial lipopolysaccharide, whereas stimulation of neutrophils results in down-regulation of CD53 expression.
Storage BufferThe reagent is provided in azide-free phosphate buffered saline (PBS) solution, pH ≈7.4; 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Bazil V, Horejsi V, Baudys M, Kristofova H, Strominger JL, Kostka W, Hilgert I: Biochemical characterization of a soluble form of the 53‑kDa monocyte surface antigen. Eur·J·Immunol. 1986·Dec; 16(12):1583‑9. <·PMID:·3493149·> | Knapp W, Dorken B, Gilks W, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (Eds): Leucocyte Typing IV. Oxford·University·Press,·Oxford. 1989; 1‑1820. <·NLM·ID:·8914679·> | Angelisová P, Vlcek C, Stefanova I, Lipoldova M, Horejsi V: The human leucocyte surface antigen CD53 is a protein structurally similar to the CD37 and MRC OX‑44 antigens.. Immunogenetics. 1990; 32(4):281‑5. <·PMID:·1700763·> | Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J·Clin·Pathol. 1993·Apr; 46(4):334‑6. <·PMID:·7684403·> | Olweus J, Lund-Johansen F, Horejsi V: CD53, a protein with four membrane‑spanning domains, mediates signal transduction in human monocytes and B cells. J·Immunol. 1993·Jul·15; 151(2):707‑16. <·PMID:·8335905·> | Olweus J, Lund-Johansen F, Horejsi V: CD53, a protein with four membrane‑spanning domains, mediates signal transduction in human monocytes and B cells. J·Immunol. 1993·Jul·15; 151(2):707‑16. <·PMID:·8335905·> | Rasmussen AM, Blomhoff HK, Stokke T, Horejsi V, Smeland EB: Cross‑linking of CD53 promotes activation of resting human B lymphocytes. J·Immunol. 1994·Dec·1; 153(11):4997‑5007. <·PMID:·7963560·> | Szollosi J, Horejsi V, Bene L, Angelisova P, Damjanovich S: Supramolecular complexes of MHC class I, MHC class II, CD20, and tetraspan molecules (CD53, CD81, and CD82) at the surface of a B cell line JY. J·Immunol. 1996·Oct·1; 157(7):2939‑46. <·PMID:·8816400·> | Mollinedo F, Martín-Martín B, Gajate C, Lazo PA: Physiological activation of human neutrophils down‑regulates CD53 cell surface antigen. J·Leukoc·Biol. 1998·Jun; 63(6):699‑706. <·PMID:·9620662·> | Puls KL, Hogquist KA, Reilly N, Wright MD: CD53, a thymocyte selection marker whose induction requires a lower affinity TCR‑MHC interaction than CD69, but is up‑regulated with slower kinetics. Int·Immunol. 2002·Mar; 14(3):249‑58. <·PMID:·11867561·> | Yunta M, Rodríguez-Barbero A, Arévalo MA, López-Novoa JM, Lazo PA: Induction of DNA synthesis by ligation of the CD53 tetraspanin antigen in primarycultures of mesangial cells. Kidney·Int. 2003·Feb; 63(2):534‑42. <·PMID:·12631118·> | Yunta M, Lazo PA: Apoptosis protection and survival signal by the CD53 tetraspanin antigen. Oncogene. 2003·Feb·27; 22(8):1219‑24. <·PMID:·12606948·> | Kim TR, Yoon JH, Kim YC, Yook YH, Kim IG, Kim YS, Lee H, Paik SG: LPS‑induced CD53 expression: a protection mechanism against oxidative and radiation stress. Mol·Cells. 2004·Feb·29; 17(1):125‑31. <·PMID:·15055538·>