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CyFlow™ CD47 FITC

CyFlow™ CD47 FITC
Antigen: CD47
Alternative Name: IAP
Clone: MEM-122
Application: Flow cytometry
Format/Fluorochrome: FITC
Laser: Blue
Target Species: Human, Non-Human Primates, Pig
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 518 nm
Excitation Maximum: 490 to 495 nm
Isotype: IgM
Order number: CA052888

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

Quantity 100 tests Volume 2 Immunogen COS-7 African green monkey kidney cells... more
CyFlow™ CD47 FITC
Quantity100 tests
Volume2
ImmunogenCOS-7 African green monkey kidney cells
Background InformationCD47 (IAP; integrin-associated protein) is an ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Binding secreted thrombospondin-1, CD47 counteracts graft vascularization. CD47 acts also as a ligand for CD172a (SIRP α; signal regulatory protein α), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion and T cell activation. CD47 is also involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing Tris buffered saline (TBS) solution, pH ≈8.0, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland·Publishing·Inc,·New·York. 1997; 1‑1342. <·NLM·ID:·9712219·> | Barazi HO, Li Z, Cashel JA, Krutzsch HC, Annis DS, Mosher DF, Roberts DD: Regulation of integrin function by CD47 ligands: Differential effects on alphavbeta 3 and alpha 4beta1 integrin‑mediated adhesion. J·Biol·Chem. 2002·Nov·8; 277(45):42859‑66. <·PMID:·12218055·> | Smith RE, Patel V, Seatter SD, Deehan MR, Brown MH, Brooke GP, Goodridge HS, Howard CJ, Rigley KP, Harnett W, Harnett MM: A novel MyD‑1 (SIRP‑1alpha) signaling pathway that inhibits LPS‑induced TNFalpha production by monocytes. Blood. 2003·Oct·1; 102(7):2532‑40. <·PMID:·12805067·> | Murata T, Ohnishi H, Okazawa H, Murata Y, Kusakari S, Hayashi Y, Miyashita M, Itoh H, Oldenborg PA, Furuya N, Matozaki T: CD47 promotes neuronal development through Src‑ and FRG/Vav2‑mediated activation of Rac and Cdc42. J·Neurosci. 2006·Nov·29; 26(48):12397‑407. <·PMID:·17135401·> | Ide K, Wang H, Tahara H, Liu J, Wang X, Asahara T, Sykes M, Yang YG, Ohdan H: Role for CD47‑SIRPalpha signaling in xenograft rejection by macrophages. Proc·Natl·Acad·Sci·USA. 2007·Mar·20; 104(12):5062‑6. <·PMID:·17360380·> | Isenberg JS, Pappan LK, Romeo MJ, Abu-Asab M, Tsokos M, Wink DA, Frazier WA, Roberts DD: Blockade of thrombospondin‑1‑CD47 interactions prevents necrosis of full thickness skin grafts. Ann·Surg. 2008·Jan; 247(1):180‑90. <·PMID:·18156939·> | Orazizadeh M, Lee HS, Groenendijk BC, Millward-Sadler J, Wright MO, Lindberg FP, Salter DM: CD47 associates with alpha 5 integrin and regulates responses of human articular chondrocytes to mechanical stimulation in an in vitro model. Arthritis·Res·Ther. 2008·Jan·10; 10(1):R4. <·PMID:·18186923·>