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CyFlow™ CD47 Pacific Blue™
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Target Species: | Human, Non-Human Primates, Pig |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | Pacific Blue™ |
| Laser: | Violet |
| Excitation Maximum: | 400 to 410 nm |
| Emission Maximum: | 455 nm |
| Isotype: | IgM |
| Antigen: | CD47 |
| Alternative Name: | IAP |
| Clone: | MEM-122 |
| Order number: | BL379138 |
For Research Use Only
Not for use in diagnostic or therapeutic procedures.
Quantity 100 tests Volume 0.4 mL Immunogen COS-7 African green monkey kidney cells... more
Product information "CyFlow™ CD47 Pacific Blue™"
| Quantity | 100 tests |
| Volume | 0.4 mL |
| Immunogen | COS-7 African green monkey kidney cells |
| Background Information | CD47 (IAP; integrin-associated protein) is an ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Binding secreted thrombospondin-1, CD47 counteracts graft vascularization. CD47 acts also as a ligand for CD172a (SIRP α; signal regulatory protein α), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion and T cell activation. CD47 is also involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
Technical Data Sheet
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