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CyFlow™ CD36 APC

CyFlow™ CD36 APC
Antigen: CD36
Alternative Name: GP3B, GPIV
Clone: TR9
Application: Flow cytometry
Format/Fluorochrome: APC
Laser: Red
Target Species: Human
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 660 nm
Excitation Maximum: 650 nm
Isotype: IgG1
Order number: CQ855808

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

Quantity 100 tests Volume 1 Immunogen Platelets Background Information CD36 (FAT;... more
CyFlow™ CD36 APC
Quantity100 tests
Volume1
ImmunogenPlatelets
Background InformationCD36 (FAT; fatty acid translocase) is an 85-113 kDa ditopic glycosylated protein that belongs to the class B family of scavenger receptors. CD36 is expressed by most resting marginal zone B cells but not by follicular and B1 B cells, and it is rapidly induced on Follicular B cells in vitro upon TLR and CD40 stimulation. CD36 does not affect the development of B cells, but modulates both primary and secondary antibody response. Similarly to glucose transporter GLUT4, CD36 is translocated from intracellular pools to the plasma membrane following cell stimulation by insulin. In mouse, CD36 is responsible for gustatory perception of long-chain fatty acids.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| van Oort MM, van Doorn JM, Bonen A, Glatz JF, van der Horst DJ, Rodenburg KW, Luiken JJ: Insulin‑induced translocation of CD36 to the plasma membrane is reversible and shows similarity to that of GLUT4. Biochim·Biophys·Acta. 2008·Jan-Feb; 1781(1‑2):61‑71. <·PMID:·18167317·> | Won WJ, Bachmann MF, Kearney JF: CD36 Is Differentially Expressed on B Cell Subsets during Development and in Responses to Antigen. J·Immunol. 2008·Jan·1; 180(1):230‑7. <·PMID:·18097024·> | Gaillard D, Laugerette F, Darcel N, El-Yassimi A, Passilly-Degrace P, Hichami A, Akhtar Khan N, Montmayeur JP, Besnard P: The gustatory pathway is involved in CD36‑mediated orosensory perception of long‑chain fatty acids in the mouse. FASEB·J. 2008·May; 22(5):145‑68. <·PMID:·18162488·>