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CyFlow™ CD31 Biotin

CyFlow™ CD31 Biotin
Antigen: CD31
Alternative Name: endoCAM, PECA1, PECAM-1
Clone: MEM-05
Application: Flow cytometry
Format/Fluorochrome: Biotin
Target Species: Human
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Isotype: IgG1
Order number: AG002104

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

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*1 Offer valid until 31/10/2017
HLDA Workshop HLDA V Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg... more
CyFlow™ CD31 Biotin
HLDA WorkshopHLDA V
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1
ImmunogenLeukocytes of a patient suffering from LGL-type leukemia
Background InformationCD31 (PECAM-1; platelet endothelial cell adhesion molecule-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.
UsageThe reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working usage is 2·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Prager E, Staffler G, Majdic O, Saemann M, Godar S, Zlabinger G, Stockinger H: Induction of hyporesponsiveness and impaired T lymphocyte activation by the CD31 receptor:ligand pathway in T cells. J·Immunol. 2001·Feb·15; 166(4):2364‑71. <·PMID:·11160294·> | Newman DK, Hamilton C, Newman PJ: Inhibition of antigen‑receptor signaling by Platelet Endothelial Cell Adhesion Molecule‑1 (CD31 requires functional ITIMs, SHP‑2, and p56(lck). Blood. 2001·Apr·15; 97(8):2351‑7. <·PMID:·11290597·> | Wilkinson R, Lyons AB, Roberts D, Wong MX, Bartley PA, Jackson DE: Platelet endothelial cell adhesion molecule‑1 (PECAM‑1/CD31 acts as a regulator of B‑cell development, B‑cell antigen receptor (BCR)‑mediated activation, and autoimmune disease. Blood. 2002·Jul·1; 100(1):184‑9. <·PMID:·12759520·> | Jackson DE: The unfolding tale of PECAM‑1. FEBS·Lett. 2003·Apr·10; 540(1‑3):41821. <·PMID:·12681475·> | Wee JL, Jackson DE: The Ig‑ITIM superfamily member PECAM‑1 regulates the "outside‑in" signaling properties of integrin alpha(IIb)beta3 in platelets. Blood. 2005·Dec·1; 106(12):3816‑23. <·PMID:·16081692·> | Woodfin A, Voisin MB, Nourshargh S: PECAM‑1: a multi‑functional molecule in inflammation and vascular biolog. Arterioscler·Thromb·Vasc·Biol. 2007·Dec; 27(12):2514‑23. <·PMID:·17872453·> | Wong MX, Hayball JD, Jackson DE: PECAM‑1‑regulated signalling thresholds control tolerance in anergic transgenic B‑cells. Mol·Immunol. 2008·Mar; 45(6):1767‑81. <·PMID:·17977600·> | Cârţână T, Săftoiu A, Gruionu LG, Gheonea DI, Pirici D, Georgescu CV, Ciocâlteu A, Gruionu G: Confocal laser endomicroscopy for the morphometric evaluation of microvessels in human colorectal cancer using targeted anti‑CD31 antibodies. PLoS·One. 2012; 7(12):e52815. <·PMID:·23285192·>