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CyFlow™ CD30 FITC
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Format/Fluorochrome: | FITC |
| Target Species: | Human |
| Regulatory Status: | RUO |
| Laser: | Blue |
| Excitation Maximum: | 490 to 495 nm |
| Emission Maximum: | 518 nm |
| Source Species: | Mouse |
| Field of Interest: | Immunophenotyping |
| Isotype: | IgG1 |
| Antigen: | CD30 |
| Alternative Name: | D1S166E, Ki-1, TNFRSF8 |
| Clone: | Ber-H8 |
| Order number: | CX511141 |
For Research Use Only
Not for use in diagnostic or therapeutic procedures.
HLDA Workshop HLDA III—WS Code A171 HLDA IV—WS Code A105 HLDA V—WS Code A042 Quantity 100... more
Product information "CyFlow™ CD30 FITC"
| HLDA Workshop | HLDA III—WS Code A171 HLDA IV—WS Code A105 HLDA V—WS Code A042 |
| Quantity | 100 tests |
| Volume | 0.4 mL |
| Immunogen | Not available |
| Background Information | CD30 is a type I transmembrane glycoprotein of the TNF receptor superfamily. CD30 was originally identified as a cell surface antigen of Hodgkins and Reed-Sternberg cells using monoclonal antibody Ki-1. The ligand for CD30 is CD30L (CD153). The binding of CD30 to CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation, and apoptotic cell death. CD30 has a critical role in the pathophysiology of Hodgkin's disease and other CD30+ lymphomas. CD30 acts as a costimulatory molecule in thymic negative selection. In addition to its expression on Hodgkin's and Reed-Sternberg cells, CD30 is also found in some non-Hodgkin's lymphomas (including Burkitt's lymphomas), virus-infected T and B cells, and on normal T and B cells after activation. In T cells, CD30 expression is present on a subset of T cells that produce Th2-type cytokines and on CD4+/CD8+ thymocytes that co-express CD45RO and the IL4 receptor. Soluble form of CD30 (sCD30) serves as a marker reflecting Th2 immune response. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
Technical Data Sheet
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