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CyFlow™ CD30 FITC

CyFlow™ CD30 FITC
Antigen: CD30
Alternative Name: D1S166E, Ki-1, TNFRSF8
Clone: Ber-H8
Application: Flow cytometry
Format/Fluorochrome: FITC
Laser: Blue
Target Species: Human
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 518 nm
Excitation Maximum: 490 to 495 nm
Isotype: IgG1
Order number: CX511141

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

HLDA Workshop HLDA III—WS Code A171 HLDA IV—WS Code A105 HLDA V—WS Code A042 Quantity 100... more
CyFlow™ CD30 FITC
HLDA WorkshopHLDA III—WS Code A171 HLDA IV—WS Code A105 HLDA V—WS Code A042
Quantity100 tests
Volume0.4
Immunogen< no data >
Background InformationCD30 is a type I transmembrane glycoprotein of the TNF receptor superfamily. CD30 was originally identified as a cell surface antigen of Hodgkins and Reed-Sternberg cells using monoclonal antibody Ki-1. The ligand for CD30 is CD30L (CD153). The binding of CD30 to CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation, and apoptotic cell death. CD30 has a critical role in the pathophysiology of Hodgkin's disease and other CD30+ lymphomas. CD30 acts as a costimulatory molecule in thymic negative selection. In addition to its expression on Hodgkin's and Reed-Sternberg cells, CD30 is also found in some non-Hodgkin's lymphomas (including Burkitt's lymphomas), virus-infected T and B cells, and on normal T and B cells after activation. In T cells, CD30 expression is present on a subset of T cells that produce Th2-type cytokines and on CD4+/CD8+ thymocytes that co-express CD45RO and the IL4 receptor. Soluble form of CD30 (sCD30) serves as a marker reflecting Th2 immune response.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Falini B, Pileri S, Pizzolo G, Dürkop H, Flenghi L, Stirpe F, Martelli MF, Stein H: CD30 (Ki‑1) molecule: a new cytokine receptor of the tumor necrosis factor receptor superfamily as a tool for diagnosis and immunotherapy. Blood. 1995·Jan·1; 85(1):41640. <·PMID:·7803786·> | Franke AC, Jung D, Ellis TM: Characterization of the CD30L binding domain on the human CD30 molecule using anti‑CD30 antibodies. Hybridoma. 2000·Feb; 19(1):43‑8. <·PMID:·10768840·> | Matsumoto K, Terakawa M, Miura K, Fukuda S, Nakajima T, Saito H: Extremely rapid and intense induction of apoptosis in human eosinophils by anti‑CD30 antibody treatment in vitro. J·Immunol. 2004·Feb·15; 172(4):2186‑93. <·PMID:·14764685·> | Berro AI, Perry GA, Agrawal DK: Increased expression and activation of CD30 induce apoptosis in human blood eosinophils. J·Immunol. 2004·Aug·1; 173(3):2174‑83. <·PMID:·15265955·> | Aalberse JA, Kapitein B, de Roock S, Klein MR, de Jager W, van der Zee R, Hoekstra MO, van Wijk F, Prakken BJ: Cord blood CD4+ T cells respond to self heat shock protein 60 (HSP60). PLoS·One. 2011; 6(9):e24119. <·PMID:·21931651·>