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CyFlow™ CD8 Alexa Fluor™ 700

CyFlow™ CD8 Alexa Fluor™ 700
Antigen: CD8
Clone: MEM-31
Application: Flow cytometry
Format/Fluorochrome: Alexa Fluor™ 700
Laser: Red
Target Species: Human
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 723 nm
Excitation Maximum: 702 nm
Isotype: IgG2a
Order number: CV618616

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

Quantity 100 tests Volume 0.4 ml Immunogen Crude thymus membrane fraction... more
CyFlow™ CD8 Alexa Fluor™ 700
Quantity100 tests
Volume0.4 ml
ImmunogenCrude thymus membrane fraction
SpecifityThe mouse monoclonal antibody MEM-31 recognizes a conformationally-dependent epitope of CD8 antigen, a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. CD8 is a disulfide-linked dimer and exists as a CD8 α/α homodimer or CD8 α/β heterodimer (each monomer approx. 32-34 kDa). The antibody does not react with formaldehyde-fixed cells; negative in Western Blotting application.
Background InformationThe CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as α/β heterodimer on majority of MHC I-restricted conventional T cells and thymocytes and as α/α homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells. Regulation of CD8 β level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 α-β but not α-α dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.1% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

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