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CyFlow™ CD4 Biotin

CyFlow™ CD4 Biotin
Application: Flow cytometry
Clonality: monoclonal
Target Species: Human
Regulatory Status: RUO
Source Species: Mouse
Field of Interest: Immunophenotyping
Isotype: IgG1
Format/Fluorochrome: Biotin
Antigen: CD4
Alternative Name: Leu3a, T4
Clone: MEM-241
Order number: CY951579
HLDA Workshop HCDM (HLDA VIII)—WS Code M241 Concentration Unit mg/mL Concentration 1... more
Product information "CyFlow™ CD4 Biotin"
HLDA WorkshopHCDM (HLDA VIII)—WS Code M241
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
Immunogen2 N-terminal domains of human CD4 fused to human IgG1 Fc
Background InformationCD4 (T4, Leu3a) is a 55 kDa single-chain transmembrane glycoprotein expressed on a subset of T lymphocytes (''helper'' T-cells) and also on monocytes, tissue macrophages and granulocytes. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1, 2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase.
UsageThe reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working usage is 0.7·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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* All prices are quoted net of the statutory value-added tax and shipping costs and possibly delivery charges, if not otherwise described

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